Authors: Lasisi A. A., Folarin M. O., Balogun S. A., Olayiwola M. A.
Science Focus, 17 (1) 2012 pp 40 – 47
Abstract
Phytochemical examination of n-hexane extract of H crepitans (root) afforded three known fatty acids: 9-octenedioic (oleic) acid (1), n-hexadecanoic acid (2) and 6-octadecenoic acid (3). EtOAc extract yielded an acyclic aliphatic ketone [n-nonadodecan-S-one (4)], a mixture of sitosterol and stigmasterol (5, 6). Structures of isolated compounds were established by EI-MS, GC-MS, IR and NMR spectroscopy. The extracts and isolated compounds were evaluated for antibacterial potencies against six pathogenic strains: Eschericia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella typhii, Proteus microbalis and Pseudomonas aueruginosa, using agar diffusion method. The extracts and
isolated compounds demonstrated broad spectrum antibacterial properties, with diameters zones of inhibition (DMZI) ranging from 5.2 ± 0.60 – 8.5 ± 1.1 rum.