Courses Code: BCH 407
Course Title: GENETIC ENGINEERING
Number of unit : 2 Units
Course Duration: Two hours per week
COURSE DETAILS
Course Coordinator: Dr. T. O Sunmonu
Email:
Office Lecturer: Room B307, College of Natural Science, Department of Biochemistry
Other Lecturer :
COURSE SYNOPSIS
Replication, transcription and translation – a brief review. Isolation and Manipulation of Nucleic acids. The genetic code and its relationship to cellular function. Gene mutation and mutagenic agents. Basic Techniques of Genetic engineering: Southern Blotting, Hybridization of Nucleic acids, Gene Probes, Polymerase chain Reaction, Gene sequencing, Gel electrophoresis of Nucleic acids. Site-directed mutagenesis. Enzymology of Genetic engineering i.e. Restriction endonucleases, ligases and others. Cloning vectors, Gene cloning, Gene banks/libraries Experession of Eukaryuotic Genes in E. Col. Cloning in plant cells and mammalian cells. Recombinant DNA techniques and applications. Uses of genetic engineering in Agriculture, Health and Industry. (Pre-requisite BCH 307,314).