Authors: J. Adetunji, I. Daniel, O.O. Oyelakin
Nigerian Journal of Genetics, Vol. 26, 2012
Abstract
Simple Sequence Repeat (SSR) markers are of particular importance in generic diversity and purity studies because of their high polymorphism and reproducibility.
The size of experiments in terms of number of samples and number of SSR markers to run, when working on genomic DNA is often limited by cost. Therefore, to identify informative SSR primers with high PIC that can be used for genetic purity analysis in most common maize cultivars in Nigeria, forty one SSR primers collected from international Institute of Tropical Agriculture, Ibadan, Nigeria were used for PCR amplifications of DNA extracted from four maize varieties commonly grown in Nigeria. The Polymorphic Information Content was in the range of 0.00 to 0.511 with a mean of 0.162, while genetic diversity detected by the primers ranged between 0.00 to 0.594 with a mean of 0.206. Sixteen SSR primers recorded PIC value above 0.3 and were identified to be informative for purity verification while 20 SSR primers were able to detect diversity among the maize varieties in Nigeria, Reason for low average PIC value was discussed.
Keywords: Maize, SSR Primers, DNA, PIC
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